Figure 6

ZD6474 in combination with UV-B alters cytoskeleton organization and extracellular MMP-9. MCF-7 and MDA-MB-468 cells were treated with ZD6474 (ZD) and/or UV-B for 24 h, and then were immunofluorescently labeled using fluorescently-labeled phalloidin (F-actin-binding protein, green) and DAPI (DNA binding dye, blue). ZD and UV-B effectively blocked cell spreading lamellipodia (white arrow) and induced thick and stress actin fiber (red dashed arrow), whereas combination treatment lead to contraction of cytoplasm and F-actin rings around the periphery of the nucleus as observed under (A) CLSM (white filled arrow head). Bars, 10 μM. ZD blocked lamellipodia and filopodia formations (black arrow), which when combined with UV-B lead to contraction of cytoplasm (black dashed arrow) and formation of membrane blebs and apoptotic bodies (white filled arrow head) in addition with drastic loss of lamellipodia-like structures as evident from the photomicrograph of the surface of breast cancer cell studied under under (B) scanning electron microscope (SEM). Bars, 10 μM. ZD6474 (ZD/Z) in combination with UV-B (R) effectively inhibits MMP-9 activity as evident from (C) Zymograph of CM of MCF-7 and MDA-MB-468 cells treated with ZD and/or UV-B.