Table 2 Identified proteins by MS/MS analysis
From: Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
Spot No. | Protein description | Gene name | Function | Accession no. | Theoretical Mr/pI | Score | No. of pep | Fold change |
|---|---|---|---|---|---|---|---|---|
1 | Annexin A2 | ANXA2 | Calcium ion binding | P07355 | 38472/7.56 | 518 | 19 | ↑5.71 ± 1.9 |
2 | Cofilin-1 | CFL1 | Rho protein signal transduction | P23528 | 18371/8.26 | 563 | 32 | ↑3.3 ± 0.9 |
3 | Apolipoprotein A-I | APOA1 | Metabolism | P02647 | 28078/5.27 | 632 | 28 | ↓2.3 ± 0.9 |
4 | Peptidyl-prolyl cis-trans isomerase A | PPIA | Metabolism | P62937 | 17881/7.82 | 335 | 19 | ↑3.5 ± 1.1 |
5 | Stathmin | STMN1 | Signal transduction | P16949 | 17171/5.77 | 84 | 4 | ↑4.9 ± 1.3 |
6 | Calcium binding protein P22 | CHP | Calcium ion binding | Q99653 | 22442/4.98 | 37 | 2 | ↑2.1 ± 0.2 |
7 | Myosin regulatory light chain 12A | MYL12A | Calcium ion binding | P19105 | 19662/4.67 | 147 | 9 | ↑2.4 ± 0.6 |
8 | Annexin A4 | ANXA4 | Calcium ion binding | P09525 | 35751/5.85 | 1024 | 39 | ↓2.2 ± 0.5 |
9 | Heat shock protein beta-1 | HSPB1 | Molecular chaperone | P04792 | 22782/5.98 | 168 | 10 | ↑2.3 ± 0.8 |
10 | Glutathione S-transferase P | GSTP1 | Apoptosis regulation | P09211 | 23224/5.44 | 342 | 22 | ↑2.1 ± 0.4 |
11 | Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | Metabolism | P04406 | 35922/8.58 | 952 | 36 | ↑2.1 ± 0.7 |
12 | Beta-enolase | ENO3 | Cell proliferation/differentiation | P13929 | 46800/7.73 | 267 | 10 | ↑2.5 ± 1.2 |
13 | Fructose-bisphosphate aldolase A | ALDOA | Metabolism | P04075 | 39288/8.39 | 406 | 25 | ↓3.9 ± 1.4 |
14 | Elongation factor Tu, mitochondrial | TUFM | Metabolism | P49411 | 49858/7.26 | 662 | 33 | ↑5.5 ± 2.1 |
15 | Cathepsin B | CTSB | Migration and invasive | P07858 | 27814/5.23 | 144 | 11 | ↑5.0 ± 1.1 |
16 | RACK1 | GNB2L1 | Metabolism | P63244 | 35055/7.60 | 205 | 5 | ↑4.7 ± 1.6 |
17 | PDZ and LIM domain protein 1 | PDLIM1 | Structrual component | O00151 | 36513/6.56 | 131 | 14 | ↑2.1 ± 0.3 |
18 | Acyl-CoA-binding protein | DBI | Metabolism | P07108 | 10038/6.12 | 220 | 5 | ↑2.3 ± 0.4 |
19 | Complement factor H-related protein 2 | CFHR2 | Metabolism | P36980 | 27896/6.52 | 61 | 10 | ↓4.3 ± 1.2 |
20 | Apoptosis regulator BAX | BAX | Apoptosis regulation | Q07812 | 21184/5.08 | 91 | 9 | ↑2.4 ± 0.8 |
21 | HLA class I histocompatibility antigen, A-25 alpha chain | HLA-A | immune response | P18462 | 38707/5.97 | 24 | 5 | ↑2.2 ± 0.5 |
22 | BMP and activin membrane-bound inhibitor homolog | BAMBI | Signal transduction | Q13145 | 25956/8.21 | 255 | 12 | ↑3.4 ± 0.9 |
23 | mRNA-capping enzyme | RNGTT | mRNA splicing | O60942 | 52533/8.3 | 44 | 9 | ↓3.1 ± 0.7 |
24 | Pyruvate kinase isozymes M1/M2 | PKM2 | Metabolism | P14618 | 57805/7.95 | 120 | 14 | ↑3.2 ± 1.2 |
25 | Proteasome activator complex subunit 2 | PSME2 | Proteolysis | Q06323 | 27515/5.44 | 327 | 17 | ↑4.9 ± 1.5 |
26 | Protein DJ-1 | PARK7 | Signal transduction | Q99497 | 20050/6.33 | 566 | 16 | ↑3.2 ± 1.3 |
27 | Transgelin | TAGLN | Metabolism | Q01995 | 22653/8.87 | 369 | 4 | ↑2.8 ± 0.6 |
28 | Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | Metabolism | P04406 | 35922/8.58 | 345 | 10 | ↑2.3 ± 0.5 |
29 | Voltage-dependent anion-selective channel protein 2 | VDAC2 | Ion channel | P45880 | 38068/6.33 | 47 | 3 | ↑3.4 ± 1.0 |
30 | Fibrinogen beta chain precursor | FGB | Metabolism | P02675 | 55928/7.95 | 55 | 14 | ↑3.5 ± 0.7 |
31 | Creatine kinase B-type | CKB | Metabolism | P12277 | 42644/5.35 | 155 | 9 | ↓2.4 ± 0.8 |