AT13387 suppressed tumor formation in nude mouse tumorigenicity assay. (A) The tumor volume of AT13387-treated mice and vehicle control mice (n = 3) were measured weekly. The solid line represented the average tumor volume of vehicle control group, which reached 1300 mm3 in week 4. The dashed line represents the average tumor volume of AT13387-treated mice which showed significant suppression of tumor formation when compared to vehicle control. (B) The mouse body weight of AT13387-treated mice and control mice during the experiment. The result showed no significant difference for mouse body weight after AT13387 drug treatment group when compared to control group.