Figure 2

Effects of kinase inhibitors on survival after radiotherapy. UT-SCC5, 24A and 40 cell lines were treated with the inhibitor overnight (16 h), irradiated with 4 Gy and changed to drug free medium 72 h after irradiation. Survival was assessed via colony counting. A) AKT inhibitor: MK-2206 (2 μM), B) SFK inhibitor: dasatinib (100 nM), C) MEK1/2 inhibitor: U0126 (10 μM), D) p38 inhibitor: SB203580 (10 μM), E) STAT5 inhibitor: 573108 (100 μM), F) STAT6 inhibitor: leflunomide (100 μM). Survival fraction shown in graph was not corrected for the effect of the inhibitor alone. Differences between survival after 4 Gy and 4 Gy + inhibitor (additive effect, first asterisks) or 4 Gy and 4 Gy + inhibitor corrected for effect of inhibitor alone (supra-additive effect, second asterisks) were tested for significance using Mann–Whitney tests. *: p < 0.05, **: p < 0.01. Error bars represent SD.