Figure 2

Induction of reporter gene expression in vitro . (a) Fluorescence intensity in TS/A cell lines stably transfected either with p21 (p21-EGFP) or constitutive promoter (CMV-EGFP) driven reporter gene GFP after 6 Gy irradiation (IR). Numbers in the bars are factors of induction of reporter gene expression or fold-induction, calculated by dividing the fluorescence obtained in the induced group by the fluorescence in the control group. The data were pooled from three independent experiments performed in 12 replicates and are presented as means + SEM; *, P < 0.05; NS, non-significant, (b) Visible and fluorescent images and surface plots of the TS/A cells stably transfected with p21 driven reporter gene GFP after 6 Gy irradiation (IR). On the surface plots fluorescence intensities are represented linearly on a rainbow scale with red being the maximum signal and black being the lowest signal.