Ets-1 increases System x
-expression and activity. A) The protein expression of the catalytic subunit of Sxc-, xCT, and the transsulfuration pathway enzyme CBS were compared in 2008 and induced 2008-Ets1 cells via Western Blot. Though the expression of CBS was not different, xCT protein levels were increased in response to Ets-1 overexpression (n = 3). B) Glutamate release was measured by AMPLEX red® fluorescence in 2008 and 2008-Ets1 cells under basal or SAS-treated conditions. Cells that overexpress Ets-1 release more glutamate into the culture medium, and SAS was an effective inhibitor of glutamate release (n = 3). C) Cell viability was measured via Crystal Violet assay following SAS treatment, which was found to decrease viability in all cell lines (n=3).