Expression pattern of 5-HT
R in HCC cells. (A) Mean ± SD relative cell viability of serum-deprived Huh7 cells cultured in the presence or absence of serotonin (5-HT) and/or SB204; compared with 5-HT (50 μM), ***P < 0.001, two-way ANOVA. (B) Western blot analysis of FOXO3a protein expression in serum-deprived Huh7 cells after incubation with SB204 (50 μM) and serotonin (50 μM) for 48h; *P<0.05, **P<0.01, t-test. (C) qRT-PCR analysis of the basal expression levels of 5-HT
R mRNA in HCC cells; *P<0.05, **P<0.01.