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Figure 1 | Molecular Cancer

Figure 1

From: The E3 ubiquitin ligases β-TrCP and FBXW7 cooperatively mediates GSK3-dependent Mcl-1 degradation induced by the Akt inhibitor API-1, resulting in apoptosis

Figure 1

API-1 decreases Mcl-1 levels (B-D) in API-1-sensitive NSCLC cell lines (A). A, The given cell lines were treated with different concentrations of API-1 ranging from 10 to 0.5 μM for 3 days. Cell numbers were then estimated with the SRB assay. Data are means ± SDs of four replicate determinations. B-D, The given cell lines were treated with different concentrations of API-1 as indicated for 12 h (B and D) or 5 μM API-1 for the indicated times (C). The cells were then harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis to detect the indicated proteins.

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Molecular Cancer

ISSN: 1476-4598