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Figure 2 | Molecular Cancer

Figure 2

From: The E3 ubiquitin ligases β-TrCP and FBXW7 cooperatively mediates GSK3-dependent Mcl-1 degradation induced by the Akt inhibitor API-1, resulting in apoptosis

Figure 2

Enforced expression of ectopic Mcl-1 (A and B), but not survivin (C and D), protects cells from API-1-induced apoptosis including caspase activation. A and B, The indicated stable transfectants were exposed to 5 μM API-1 for 24 h and then harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis to detect caspase cleavage and Mcl-1 expression (A) and for detection of apoptosis (i.e., annexin V-positive cells) with flow cytometry (B). P, parental; V, vector. C, The indicated cell lines were exposed to the given concentrations of API-1 for 24 h and then harvested for preparation of whole cell protein lysates and subsequent Western blot analysis. D, The indicated H157 stable transfectants were treated with different concentrations of API-1 as indicated for 3 days. Cell numbers were estimated with SRB assay. The data are means ± SDs of four replicate determinations. CF, cleaved fragment.

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Molecular Cancer

ISSN: 1476-4598