Figure 5

MEF2D promotes muscle differentiation in ARMS cells. A. The expression of MEF2D was confirmed by RNA and western blot (B) analysis in RH30 cells stably transfected with an expression construct for MEF2D or a vector only control. C. MEF2D expression activates muscle specific genes. Quantitative gene expression analysis of the indicated endogenous muscle specific genes in RH30 cells stably transfected with MEF2D or vector only expression constructs. D. MEF2D expression activates p21 (CDKN1A) expression at the level of RNA and protein (E). F. MEF2D promotes myosin heavy chain expression. Western blots were probed with antibodies against myosin heavy chain (MyHC) and GAPDH. G. MEF2D promotes myofiber formation. RH30 cells transfected with MEF2D or vector control were immunostained with myosin heavy chain antibodies. DAPI staining is also shown. Fluorescent microscopy images were taken at 200X and scale bars represent 10 μms. H. MEF2D expression upregulates myogenin. Western blot was probed with antibodies against myogenin and GAPDH.