Figure 4

Immunohistochemical analysis of lung tissue after treatment with selective CXCR2 inhibitor. (A) Labeling with P-40 Ab to detect infiltrating neutrophils in non-tumoral area and in tumoral area in the lung of CC-LR mice with or without SBZ treatment in the presence of NTHi (20× magnification, scale bar = 50 mm, applicable to all panels). (B) Quantitative analysis of P-40 positive staining (mean ± SE; * = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus SBZ treatment in non-tumoral area; # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus SBZ treatment in tumoral area). (C) Representative photomicrographs of Ki-67 positive cells in lung tissue of CC-LR mice with or without SBZ treatment in presence or absence of NTHi (40× magnification, scale bar = 50 mm, applicable to all panels). (D) Quantitative analysis of Ki-67 positive staining (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus SBZ treatment, # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus SBZ treatment). (E) Representative photomicrographs of CD31 positive cells in lung tissue of CC-LR mice with or without SBZ treatment in presence or absence of NTHi (20× magnification, scale bar = 50 mm, applicable to all panels). (F) Quantitative analysis of CD31 positive staining (mean ± SE; * = P ≤ 0.05 for CC-LR vs CC-LR plus SBZ treatment, # = P ≤ 0.05 for CC-LR with NTHi exposure vs CC-LR with NTHi exposure plus SBZ treatment).