Figure 6

MiR-204-5p inhibits the growth of mouse xenografts bearing human endometrial carcinoma cells. (A) Ishikawa^TrkB cells were transfected with lentiviruses encoding miR-204 or its scrambled control (NC). MiR-204 was detected by Taq Man PCR and normalized against U6B. Data are expressed as mean ± SD of at least three independent experiments. ** P < 0.01. (B) Tumor growth curve in nude mice bearing Ishikawa^TrkB cells transfected with lentiviruses encoding miR-204 or its scrambled control. Data are expressed as mean ± SD of 8 mice. * P < 0.05. (C) Photograph of tumor xenografts. (D) Tumor weights are expressed as mean ± SD of tumors from panel C. *P < 0.05. (E) Representative H&E staining histopathology of miR-204 NC and miR-204 tumor tissues in mice (left panels). MiR-204 expression was detected by in situ hybridization (second panels), and TrkB, p-STAT3, Ki 67 and PCNA expression was detected by immunohistochemistry (right panels) (magnification, 200×). Results are representative of three independent experiments.