Figure 4

FLOT1 is essential for breast cancer cell proliferation and invasion, and involved in the miR-124 -induced inhibition of cell proliferation and migration. A, Analysis of protein expression by western blot, FLOT1 protein is reduced by small interfering RNA (FLOT1-siRNA) in MDA-MB-231 and T47D cells. B, MTT assay show that cell proliferation is inhibited in MDA-MB-231 and T47D cells after transfection of FLOT1-siRNA compared with scrambled sequence (Ctrl-siRNA). C, Cell invasion of MDA-MB-231 and T47D cells after knockdown of FLOT1 are evaluated by Matrigel invasion chamber. D, MTT assay shows cell proliferation in MDA-MB-231 and T47D cells co-transfected with miR-124 and wt/mut 3′-UTR-FLOT1 compared with scrambled miRNA mimics (miR-Ctrl). E, Cell invasion ability of MDA-MB-231 and T47D cells which co-transfection of miR-124 and wt/mut 3′-UTR-FLOT1 were evaluated by Matrigel invasion chamber compared with scrambled miRNA mimics (miR-Ctrl). *, P < 0.05 compared with control.