CD133 and invasiveness of CD133highcells. (A) Western blot analysis with the anti-CD133 antibody of mmunoprecipitates with anti-CD133 antibody from CD133high cells transfected with specific siRNAs (CD133 siRNAs). A non-silencing scramble siRNAs was used as a control (ctrl siRNAs). Lysates from the same cells were analyzed for β-tubulin content, as internal control of processed proteins. (B) Dynamic monitoring of invasion through Matrigel using the xCELLigence system of transfected cells. Error bars indicate ± SD. (C) Immunochemical analysis with the indicated antibodies of lysates from CD133high cells in which CD133 expression was down-modulated. Tubulin was blotted as a loading control. The data are representative of three separate experiments.