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Figure 5 | Molecular Cancer

Figure 5

From: Reduced annexin A6 expression promotes the degradation of activated epidermal growth factor receptor and sensitizes invasive breast cancer cells to EGFR-targeted tyrosine kinase inhibitors

Figure 5

Effects of AnxA6-depletion on lysosomal degradation and recycling of EGFR. A-D) Control BT-EV (A and B) or AnxA6-depleted BT-A6sh5 (C and D) cells were pretreated overnight with or without CLQ (50 μg/ml) during serum-starvation. Cells were briefly rinsed in HBSS, treated with EGF for up to 90 min in the presence or absence of CLQ respectively. Total and Y1068-EGFR were examined by western blotting. E) Serum-starved AnxA6-depleted (BT-A6sh5) and control (BT-EV) cells grown on glass cover slips were treated with EGF for 5 or 90 min. The cells were fixed in 3.7% PBS-buffered formaldehyde and processed for indirect immunofluorescence as described in Figure 1D using anti-pY1068 EGFR (green) and either LAMP1 or Rab11 (red). Nuclei were stained with DAPI.

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Molecular Cancer

ISSN: 1476-4598