Figure 2

CD98hc expression is not affected by integrins. (A) Western blot (under reducing conditions). Matrix independent CD98hc expression, 85 kDa. Loading control = beta actin, 45 kDa. FN = cells seeded on fibronectin for or PDL (poly – D – lysine). Representative blots are demonstrated. 4 separate experiments. (B) FACS analysis demonstrate no alteration in beta 1 (upper pattern) or beta 3 (lower pattern) integrin adhesion receptors in low and high CD98hc expressing Caki2 cells.