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Figure 1 | Molecular Cancer

Figure 1

From: Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation

Figure 1

Increased association of Timp1 on the cell surface along melanoma progression. A. mRNA levels of Timp1 were determined by real time PCR. B. Membrane-enriched extracts were separated on 15% polyacrylamide gel SDS/PAGE and transferred to a PVDF membrane. The membrane was incubated with polyclonal antibody specific to Timp1. The constitutive expression of β-actin was used as endogenous control. C. The supernatants of cell cultures were collected and lyophilized. The presence of soluble Timp1 was identified by Western Blotting. D. Conditioned media (10 μg) from melan-a, 4C, 4C11- and 4C11+ cell lines were evaluated for MMPs activity. E. The same cell lines were maintained in suspension for 96 hours and viable cells were estimated using MTT. Experiments were always performed in quadruplicates. ma: murine non-tumorigenic melanocyte lineage; 4C: pre-malignant melanocytes; 4C11-: non-metastatic melanoma cells; 4C11+: metastatic melanoma cells. **p < 0.01, ***p < 0.001, ****p < 0.0001.

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Molecular Cancer

ISSN: 1476-4598