Table 1 Primer sets used in site-directed mutagenesis of the E-cadherin promoter
From: The Wilms’ tumor gene (WT1) regulates E-cadherin expression and migration of prostate cancer cells
Site mutated | Sequence |
|---|---|
-15 bp (a) | F 5′ –CTGGCTGCAGCCACGCATTT CCTCTCAGTGGCGTC-3′ |
R 5′ – GACGCCACTGAGAGGAAA TGCGTGGCTGCAGCCAG-3′ | |
-51bp (a) | F 5′- CAATCAGCGGTACGGTTT GCGGTGCTCCGGGGC-3′ |
R 5′- GCCCCGGAGCACCGCAAA CCGTACCGCTGATTG-3′ | |
-146bp (b) | F 5′- CGTCTATGCGAGGCCGTTTGTT CGGGCCGTCAGCTCCG-3′ |
R 5′- CGGAGCTGACGGCCCGAACAAA CGGCCTCGCATAGACG-3′ |