Table 2 TaqMan and Sybergreen qRT-PCR primer sets used for expression analyses
From: The Wilms’ tumor gene (WT1) regulates E-cadherin expression and migration of prostate cancer cells
Functional Class | Gene | Sequence |
|---|---|---|
Housekeeping gene | 18S normalizer (a) | Hs9999990_s1 |
GAPDH (b) | F 5′CCATCACCATCTTCCAGGAG 3′ | |
R 5′ GGATGATGTTCTGGAGAGCG | ||
Transcription Factor | Wt1(mouse) (b) | F 5′ TGGTCTGAGCGAGAAAACCT 3′ |
R 5′TCTTCCGAGGCATTCAGGAT 3′ | ||
WT1(human) (b) | F 5′GAGAGCCAGCCCGCTATTC 3′ | |
R 5′ CATGGGATCCTCATGCTTG 3′ | ||
Snail(human) (b) | F 5′ ACCCCACATCCTTCTCACTG 3′ | |
5′ TACAAAAACCCACGCAGACA 3′ | ||
Adhesion molecule | E-cadherin (a) | Hs00170423_m1 |