Ad-eIF5A1 and Ad-eIF5A1
infection activate MAPK/SAPK pathways. A549 lung carcinoma cells were infected with adenovirus expressing eIF5A1 (A) or the non-hypusinable mutant eIF5A1K50A (B) at increasing multiplicities of infection (MOI). A) A549 cells were treated with Ad-eIF5A1 at an MOI of 5, 10, 25, 50 or 80 and Ad-LacZ at an MOI of 80. B) A549 cells were treated with Ad-eIF5A1K50A at an MOI of 5, 25, 50 or 80 and Ad-LacZ at an MOI of 80. Twenty-four hours later, a subset of infected cells were treated with 10 μM of the MEK inhibitor U1026. A, B) Forty-eight hours after infection, cell lysate was harvested and used for western blot analysis using antibodies to eIF5A1 or the MAPK/SAPK pathway. The data is representative of three independent experiments. Quantification of expression of phosphorylated p38 and phosphorylated p42/p44 MAPK relative to expression of un-phosphorylated total protein from (A) is shown in (C) and (D), respectively.