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Figure 6 | Molecular Cancer

Figure 6

From: Role of p38 and JNK MAPK signaling pathways and tumor suppressor p53 on induction of apoptosis in response to Ad-eIF5A1 in A549 lung cancer cells

Figure 6

Induction of apoptosis by Ad-eIF5A1 infection is dependent on p38 and JNK activity. A549 lung carcinoma cells were infected with adenovirus expressing either LacZ or eIF5A1. Four hours after infection, the media was replaced with media containing either DMSO, 10 μM of the MEK inhibitor U1026, 10 μM of the p38 inhibitor SB203580, 10 μM of the JNK inhibitor SP600125 (JNK), 10 μM of SB203580 and SP600125 (SB + JNK), or 30 μM of the p53 inhibitor pifithrin-α. Forty-eight hours later, the cells were harvested and the percentage of cells undergoing apoptosis was determined by Annexin/PI staining and flow cytometry. The data shown is the mean of 3 independent experiments. Statistical significance in comparison to Ad-eIF5A1 infected cells treated with DMSO is indicated (* p < 0.05; ** p < 0.01; *** p < 0.001).

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Molecular Cancer

ISSN: 1476-4598