Figure 1

CARM1 expression and sub-cellular localization of across molecular and histological tumor subtypes. A. Representative CARM1 IHC stains for Adjacent-normal (top row) and tumor (bottom row) tissue cores. There is a diverse range of both cytoplasmic and nuclear expression levels for each pathological and histological subtype. The specific histological subtype and nuclear receptor status are indicated on respective images. The CARM1 nuclear/cytoplasmic scores are indicated in parentheses (e.g. (3/2) = nuclear score of 3 and cytoplasmic score of 2). B. Nuclear and Cytoplasmic CARM1 expression tumor score averages, grouped by histological tumor types (top) and molecular tumor subtypes (bottom). Histological subtypes appear to show very little variation in CARM1 expression, with small variations in localization in the invasive and metastatic tumor categories. Normal tissue indicates CARM1 is usually highest in the nucleus. For the molecular subtypes, there is a wide range of variation among the categories. HER2+ tumors have the highest relative expression of CARM1, while basal-like subtypes have the most divergent localization between nucleus and cytoplasm, with the majority of the protein residing in the cytoplasm, contrary to what is seen in normal tissues overall. Asterisks denote findings that were statistically significant and are outlined in Table 1. Error bars are shown to denote Standard Deviation(1).