Figure 3
From: Thymoquinone attenuates tumor growth in ApcMin mice by interference with Wnt-signaling
TQ decreases nuclear c-myc protein in polyps of APCMinmice. IRS of nuclear c-myc expression from polyps (n=16) of untreated, TQ-low, TQ-high, or piroxicam treated APCMin mice (n=4; A). ANOVA, Dunnett, 2-sided was used to compare the different treatment groups to the untreated group; **p<0.01, ***p<0.001. Every dot in the graph represents a single polyp analyzed for nuclear c-myc intensity as follows: 0, negative staining; 1+, weak staining; 2+, moderate staining. Treatment with TQ-low or TQ-high significantly decreased nuclear c-myc within the polyps. Representative image for untreated (B) and TQ-high treated (C) mice. Image D shows the negative control sample without the primary antibody (D). Magnification: 100× (left panel), 200× (right panel).