Figure 3From: Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cellsATRA promotes recruitment of RARα to the plasma membrane . A549 cells were serum-starved and treated with 5 μM of ATRA for the times indicated. Then cells were fixed and incubated with anti-RARα and anti-Akt followed by incubation with anti-mouse Alexa Fluor 532 and Alexa Fluor 647, respectively, as described in Materials and Methods. Finally, the cells were analyzed by confocal microscopy.Back to article page