Figure 2

Rapamycin treatment results in the redistribution of YY1 from the nucleus to cytoplasm in AML cells. Immunofluorescene staining was used to detect the localization of YY1 in AML cells treated with rapamycin (0–40 nM) for 24 hours. (A) FITC green signals for YY1 were detected using a filter with excitation range of 488 nm and (B) propidium iodide red signals for nuclear DNA using a filter with excitation at 535 nm. (C) Overlay of YY1 and DNA staining, demonstrating nuclear localization of YY1 in AML cells treated with rapamycin. To show staining specificity, control cells were stained without primary antibody. (D) Rapamycin treatment significantly decreased YY1 in the nuclear fraction. Lamin B was used as a nuclear marker and GAPDH as cytoplasmic marker.