Figure 3
From: Novel mechanism of regulation of fibrosis in kidney tumor with tuberous sclerosis
Tuberin regulates αSMA expression in human cells. (A) Immunoblot analysis shows that downregulation of tuberin with siRNA directed against TSC2 results in an increased in αSMA protein expression in human renal embryonic epithelial cells (HEK293) in cells grown in normal glucose or pretreated with high glucose (25 mM) for 24 h. (B) Overexpression of tuberin decreases αSMA expression. HEK293 cells were infected with adenovirus 6.01 expressing tuberin complementary DNA. An adenovirus vector expressing protein (Ad-GAL) was used as a control. Overexpression of tuberin results in decreased αSMA protein expression in cells grown in normal glucose or pretreated with high glucose (25 mM) for 24 h. GAPDH was used as a loading control.