Figure 4

Molecular characterization of fludarabine surviving cells. A, schematic representation of the miR-485-3p/NF-YB/Top2α axis in cells surviving the treatment of fludarabine. B, western blot analysis and quantification of NF-YB after fludarabine treatment. QRT-PCR analysis (C) and western blot analysis and quantification (D) of Top2α after fludarabine treatment. E, schematic representation of miR-485-3p/NF-YB-p53/MDR1 or CCNB2 axis in cells surviving treatment of fludarabine. F, representative immunoblot of p53 levels in cellular lysates of DU-145 cells treated (+) or untreated (-) with fludarabine (INPUT), in immunoprecipitates (IP) with anti NF-YB and anti-FLAG antibodies. G, relative expression level of CCNB2 and MDR1 in DU-145 cells treated with fludarabine versus untreated cells. All data were shown as S.D. from three independent experiments (*P<0.05, **P<0.01, ***P<0.001, unpaired t-test).