Table 3 Primers for amplifying 11 exons of BCAT1
Primer | Sequence (5’-3’) | Product size (bp) |
|---|---|---|
Exon 1 | F-GGGGAGCAGCCTTAGTGT | 456 |
R-GAGTGGAGGTTAAACCGAAA | ||
Exon 2 | F-TACCCACCTGCATTTACTT | 583 |
R-TCAACGTGCTTTGTTTCTC | ||
Exon 3 | F-TAATCTAGCCAGCGAATG | 311 |
R-GTACCCACAGTGAAGTGC | ||
Exon 4 | F-GATGAACGCCCATAGGAA | 251 |
R-CCGTGACCCGTTACATTA | ||
Exon 5 | F-ATTGCCACATTGTGAGAAA | 417 |
R-GTATGGTAAGAGGTAGGGA | ||
Exon 6 | F-AAGTATGGTAATAGCTCCTG | 352 |
R-ATGGCACTAACTAAATGGTC | ||
Exon 7 | F-GGGGATGAAGTATGTTTG | 250 |
R-GTCTTTCTGGTCCTGTTG | ||
Exon 8 | F-ATGCCTAATGTAGTGAAAG | 478 |
R-ACAGACTTGGGAAGTTAA | ||
Exon 9 | F-GCCACTTCCAGCTTTCCC | 385 |
R-GCATCTTGGGTCTGGGTC | ||
Exon 10 | F-CTTCAGTGGAATTGCCTTAG | 375 |
R-TTTCCCATTTCTGCTTTG | ||
Exon 11 | F-TCAAAGCAGAAGCGAACC | 251 |
R-GTAGCCAAAGAAATCTATCACA |