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Table 3 List of miRNAs over-expressed in prom1-exo compared with parental FEMX-I cells

From: Biochemical and biological characterization of exosomes containing prominin-1/CD133

miRNA Ct FEMX-Cells Ct FEMX-Exosomes DCt Fold-charge
hsa-miR-216b >40 15.81 24.2 19138839.3
hsa-miR-889 37.33 15.86 21.47 2910427.1
hsa-miR-4307 >40 22.51 17.5 184083.4
hsa-miR-4272 >40 22.99 17 131983.7
hsa-miR-203 >40cyclcs 23.82 16.2 74244.7
hsa-miR-4289 23.34 8.67 14.66 25944.3
hsa-miR-3149 22,7 8.77 13.94 15746.0
hsa-miR-203 26.41 13.69 12.72 6769.4
hsa-miR-3145 21.19 10.53 10.66 1622.1
hsa-miR-1911 >40 29.72 10.3 1243.3
hsa-miR-513a-3p >40 29.84 10.2 1144.1
hsa-miR-3916 >40 30.52 9.48 714.1
hsa-miR-886-3p >40 32.31 7.69 206.5
hsa-miR-1182 22.77 15.91 6.86 115.1
hsa-miR-3613-5p >40 33.69 6.31 79.3
hsa-let-7i 22.95 17.21 5.73 53.2
hsa-miR-3132 16.50 11.49 5.01 32.2
hsa-miR-3914 24.75 20.39 4.36 20.5
hsa-miR-3618 28.56 24.35 4.21 18.5
hsa-miR-1307 21.87 17.96 3.91 15.0
hsa-miR-3614-3p 21.90 19.15 2.75 6.7
hsa-miR-519c-3p 22.59 20.22 2.3k 5.2
hsa-miR-3160 17,61 15.28 2.33 5.0
hsa-miR-3153 11.48 9.53 1.96 3.9
hsa-miR-4278 18.94 16.99 1.95 3.9
hsa-miR-3646 I.58 15.80 1.79 3.5
hsa-miR-3926 17.47 15.72 1.75 3.4
hsa-miR-515-5p 28.37 26.69 1.68 3.2
hsa-miR-3169 14.33 12.67 1.66 1.2
hsa-miR-10a 31.87 30.21 1.66 3.2
hsa-miR-140-5p 26.92 25.37 1.55 2.9
hsa-miR-3148 18.74 17.56 1.18 2.3
hsa-miR-4271 17.56 16.48 1.08 2.1
hsa-miR-627 23.07 22.00 1.07 2.1
hsa-miR-548d-3p 29.69 28.66 1.03 2.0
hsa-miR-3613-3p 22.09 21.19 0.90 1.9
hsa-miR-481 26.49 25.64 0.85 1.8
hsa-miR-571 20.81 19.97 0.84 1.8
hsa-miR-4274 19.93 19.15 0.79 1.7
hsa-miR-4277 21.41 20.79 0.62 1.5
hsa-miR-3686 15.41 14.81 0.61 1.5
hsa-miR-3074 21.65 21.10 0.54 1.5
hsa-miR-95 24.90 24.45 0.46 1.4
hsa-miR-590-3p 26.81 26.49 0.32 1.2
hsa-miR-525-5p 23.20 22.90 0.30 1.2
hsa-miR-548g 26.97 26.69 0.28 1.2
hsa-miR-365 25.46 25.18 0.28 1.2
hsa-miR-525-3p 23.23 22.94 0.28 1.2
hsa-miR-320d 21.97 21.93 0.04 1.0
  1. Total RNA from FEMX-I cells and exosomes was prepared with Qiazol extraction followed by poly-A tailing reactions and miRNA cDNA synthesis. 250 ng of cell’s total RNA and of exosomes’ RNA were used in cDNA synthesis. cDNA synthesis were carried out simultaneously and equal volume of cDNA synthesis reaction product was used in the subsequent real-time qPCR reactions. 1058 miRNAs were investigated. The DeltaCt (DCt) values for each miRNA-specific prom1-exo cDNA greater than 0.01 were listed.