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Table 3 List of miRNAs over-expressed in prom1-exo compared with parental FEMX-I cells

From: Biochemical and biological characterization of exosomes containing prominin-1/CD133

miRNA

Ct FEMX-Cells

Ct FEMX-Exosomes

DCt

Fold-charge

hsa-miR-216b

>40

15.81

24.2

19138839.3

hsa-miR-889

37.33

15.86

21.47

2910427.1

hsa-miR-4307

>40

22.51

17.5

184083.4

hsa-miR-4272

>40

22.99

17

131983.7

hsa-miR-203

>40cyclcs

23.82

16.2

74244.7

hsa-miR-4289

23.34

8.67

14.66

25944.3

hsa-miR-3149

22,7

8.77

13.94

15746.0

hsa-miR-203

26.41

13.69

12.72

6769.4

hsa-miR-3145

21.19

10.53

10.66

1622.1

hsa-miR-1911

>40

29.72

10.3

1243.3

hsa-miR-513a-3p

>40

29.84

10.2

1144.1

hsa-miR-3916

>40

30.52

9.48

714.1

hsa-miR-886-3p

>40

32.31

7.69

206.5

hsa-miR-1182

22.77

15.91

6.86

115.1

hsa-miR-3613-5p

>40

33.69

6.31

79.3

hsa-let-7i

22.95

17.21

5.73

53.2

hsa-miR-3132

16.50

11.49

5.01

32.2

hsa-miR-3914

24.75

20.39

4.36

20.5

hsa-miR-3618

28.56

24.35

4.21

18.5

hsa-miR-1307

21.87

17.96

3.91

15.0

hsa-miR-3614-3p

21.90

19.15

2.75

6.7

hsa-miR-519c-3p

22.59

20.22

2.3k

5.2

hsa-miR-3160

17,61

15.28

2.33

5.0

hsa-miR-3153

11.48

9.53

1.96

3.9

hsa-miR-4278

18.94

16.99

1.95

3.9

hsa-miR-3646

I.58

15.80

1.79

3.5

hsa-miR-3926

17.47

15.72

1.75

3.4

hsa-miR-515-5p

28.37

26.69

1.68

3.2

hsa-miR-3169

14.33

12.67

1.66

1.2

hsa-miR-10a

31.87

30.21

1.66

3.2

hsa-miR-140-5p

26.92

25.37

1.55

2.9

hsa-miR-3148

18.74

17.56

1.18

2.3

hsa-miR-4271

17.56

16.48

1.08

2.1

hsa-miR-627

23.07

22.00

1.07

2.1

hsa-miR-548d-3p

29.69

28.66

1.03

2.0

hsa-miR-3613-3p

22.09

21.19

0.90

1.9

hsa-miR-481

26.49

25.64

0.85

1.8

hsa-miR-571

20.81

19.97

0.84

1.8

hsa-miR-4274

19.93

19.15

0.79

1.7

hsa-miR-4277

21.41

20.79

0.62

1.5

hsa-miR-3686

15.41

14.81

0.61

1.5

hsa-miR-3074

21.65

21.10

0.54

1.5

hsa-miR-95

24.90

24.45

0.46

1.4

hsa-miR-590-3p

26.81

26.49

0.32

1.2

hsa-miR-525-5p

23.20

22.90

0.30

1.2

hsa-miR-548g

26.97

26.69

0.28

1.2

hsa-miR-365

25.46

25.18

0.28

1.2

hsa-miR-525-3p

23.23

22.94

0.28

1.2

hsa-miR-320d

21.97

21.93

0.04

1.0

  1. Total RNA from FEMX-I cells and exosomes was prepared with Qiazol extraction followed by poly-A tailing reactions and miRNA cDNA synthesis. 250 ng of cell’s total RNA and of exosomes’ RNA were used in cDNA synthesis. cDNA synthesis were carried out simultaneously and equal volume of cDNA synthesis reaction product was used in the subsequent real-time qPCR reactions. 1058 miRNAs were investigated. The DeltaCt (DCt) values for each miRNA-specific prom1-exo cDNA greater than 0.01 were listed.
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Molecular Cancer

ISSN: 1476-4598