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Figure 1 | Molecular Cancer

Figure 1

From: Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

Figure 1

The expression of GLI1 and HIF-1α in human pancreatic cancer cell lines. (A) The expression of GLI1 at protein level in MIAPaCa-2, AsPC-1, PANC-1, BxPC-3, CFPAC-1 and SW1990 was estimated by Western blot. 100 ug of cellular proteins were separated on a 10% SDS-PAGE gel, and transferred onto a PVDF membrane. Antibodies specific for GLI1 were used to probe the immunoblots. The blots were then re-probed with β-actin antibody as a loading control. (B&C) The expression of GLI1 and HIF-1α at mRNA level was evaluated by qRT-PCR. The expression of each target gene was quantified using GAPDH as a normalization control. Results are representative of three independent experiments. Column: mean; bar: SD.

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Molecular Cancer

ISSN: 1476-4598