Figure 1

Effect of loss of Id4 on mouse prostate development. A and B – Immuno-histochemical analysis of Id4 expression in the wild type mouse prostate. Panel B is the enlarged version of the box represented in Panel A. Id4 expression is primarily localized to epithelial cell nuclei. Red, green and yellow arrow heads represent cells expressing high, low or no Id4 respectively. C: Lack of Id4 expression in Id4-/- prostates. The scale bars are 100um. D: The relative size of the genital tract (excluding testis and epididymis) of the wild type (Id4+/+) and mutant (Id4-/-) mice. The representative image of three different tracts demonstrating clear size differences is shown. The bottom panel represents the size of the seminal vesicle from the Id4+/+, Id4+/- and Id4-/- mice. E: Average number of tubules and tubule diameter per cross section in the wild type and Id4 -/- prostates. The number of tubules were counted in each section (n = 100 serial cross sections for Id4+/+, n = 76 for Id4+/- and n = 54 for Id4-/, proximal to distal) at 50× and their mean ± SEM is indicated as open bars. The black bars represent average tubule diameters (±SEM) of the number of tubules counted in each of the serial sections at 50×. (*** P < 0.001, NS: non-significant).