Figure 3
Expression levels of TLR2 are inversely correlated with miR-143 in CRC tissues and cell lines. (A) Expression of miR-143 was determined using real-time PCR in 79 CRC tissue samples, and the correlation of TLR2 expression levels with miR-143 in CRC tissues was evaluated using nonparametric tests (R2 = 0.4486; p = 0.000). (B) Expression of miR-143 was determined by northern blotting in 5 normal colon epithelial cells and 4 CRC cell lines (SW480, LS174T, SW620, and HCT116). (C) Expression of miR-143 was determined in 79 CRC tissues (grade1 n = 18; grade2 n = 20; grade3 n = 41) by real-time PCR. The statistical method analysed the correlation between the expression of miR-143 and tumour pathological grade. (D) Expression of miR-143 was determined in 79 CRC tissues (N0 n = 28; N1 n = 31; N2 n = 20) by real-time PCR. The statistical method analysed the correlation between the expression of miR-143 and tumour lymph node metastasis. (E) Higher overall survival of patients with high miR-143 levels (above median; n = 38) compared to patients with low miR-143 levels (below median; n = 41). (***p < 0.001; **p < 0.01; *p < 0.05).