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Figure 1 | Molecular Cancer

Figure 1

From: Late developing mammary tumors and hyperplasia induced by a low-oncogenic variant of mouse mammary tumor virus (MMTV) express genes identical to those induced by canonical MMTV

Figure 1

Detection of the Mtv-1/NIV virus in Balb/c NIV mammary tumors. (A) PCR amplification of a 3.0 Kb fragment from genomic DNA isolated from mammary tumors and liver tissues by Polymerase Chain Reaction (PCR) with MMTV (C3H)-specific primers. The MMTV (C3H) 3.0 Kb PCR product was detected in DNA isolated from Balb/c NIV mammary tumors, C3Hf mammary tumors and C3Hf liver tissue (positive controls) but not in Balb/c liver. Primers designed to the region 12918–1303 of Chromosome 1 were used as a loading control and to confirm genomic DNA quality. (B and C) Electron micrographs of ultrathin sections taken from mammary tumors of a C3H (MMTV) (B) and C3Hf (NIV) (C) mice show the presence of B-type retrovirions are abundant in both tumor types. Micrographs were taken of representative viral particles from ultrathin sections of OSO4 (osmium teroxide)-fixed tumor tissues, which had been stained with uranyl acetate and lead citrate to enhanced their electron density. The micrographs were taken on a Philips 300 electron microscope equipped with a camera. Scale bars = 200 nm.

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Molecular Cancer

ISSN: 1476-4598