Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Tylophorine, a phenanthraindolizidine alkaloid isolated from Tylophora indica exerts antiangiogenic and antitumor activity by targeting vascular endothelial growth factor receptor 2–mediated angiogenesis

Figure 3

Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.

Back to article page

Molecular Cancer

ISSN: 1476-4598