Figure 7
Effect of tylophorine on tumor growth and VEGFR2 phosphorylation in EAC tumor model. 15 × 106 EAC cells/mouse were injected s.c. into 5–6 week old Swiss albino mice. After solid tumors grew to ~ 100 mm3, the mice were i.p. treated with tylophorine (7.5 mg/kg bw). Tumor growth was measured with calipers every fifth days using the formula: Tumor volume(mm3) = (width)2 × (length) × π/6. Tylophorine treatment decreases (A) tumor volume (B) body weight. Data were presented as means ± SEM, n = 15. (C) Representive images of solid tumor lump shows tylophorine treated group is significantly smaller than those in the control group. (D) The tumor tissue was removed from mice at 30 days after treatment and weighed. (E) Immunohistochemical staining of tumor tissue (n = 6) with antibodies against P-VEGFR2, P-ERK, P-Akt and CD31. (F) % MVD was determined by selecting the blood vessel (CD31) area per field in selected vascularized areas divided by the whole area. Data were presented as means ± SEM, n = 6. ***p < 0.001 versus control group.