Phenotypic properties of PDAC cell lines. A) FGFR3 expression in the PDAC cell lines was determined by western-blot. FGFR3 expression was the highest in BxPC-3 cells and the lowest in Mia PaCa-2 cells. Membranes were reprobed for GAPDH to test equivalent loading. B) FGFR3 splice variants relative mRNA level were determined by RT-qPCR with specific primers and normalized with RPLP0 used as a mRNA expression internal reference. C) Epithelial E-cadherin and mesenchymal vimentin markers mRNA levels were determined by RT-qPCR. Capan-2 and BxPC-3 cells were epithelial, whereas the Mia PaCa-2 cell line was more mesenchymal. PANC-1 cells displayed an intermediate phenotype. D) Light microscope pictures of the four tested cell lines (original magnification ×40). PANC-1 presented an epithelial phenotype despite the strong expression of vimentin.