Loss of PTEN results in increased expression of CXCL12 and CXCR4 in murine prostate epithelial cells. A) Cell lysates were collected from PTEN+/+, PTEN+/−, and PTEN−/− cells and analyzed by Western blot for PTEN. B) mRNA expression levels of CXCL12 and its receptor, CXCR4, in PTEN+/+, PTEN+/−, and PTEN−/− cells were analyzed by quantitative RT-PCR. C, D) PTEN−/− cells were treated for 18 hours with increasing concentrations of Akt Inhibitor IV. Protein and mRNA expression levels were analyzed by Western blot (C) and quantitative RT-PCR (D), respectively. E) PTEN−/− cells were pretreated with or without 10 μM Akt Inhibitor IV; 2x105 cells were then plated on Matrigel coated inserts, allowed to invade for 24 hours, and stained with Crystal Violet. Total number of migrated cells was counted under 10X magnification in five fields. Assay was performed in triplicate. *: p < 0.005.