mRNA expression changes of ERα , HDAC1 and DNMT1 in mice breast tumors. Left panel, MDA-MB-231 breast tumor xenografts (Protocol 1); right panel, C3(1)-SV40 Tag transgenic mouse tumors (Protocol 2). A) mRNA expression of ERα. B) mRNA expression of DNMT1. C) mRNA expression of HDAC1. mRNA expression in breast tumors was analyzed by quantitative real-time PCR. GE and/or TAM treatment were described in Materials and methods. All the analyses in tumor samples were performed at the termination of the experiment. Columns, mean; Bars, SD; *, P < 0.05, significantly different from control; * * P< 0.01, significantly different from control.