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Figure 2 | Molecular Cancer

Figure 2

From: Histone acetyltransferase PCAF Up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling

Figure 2

Overexpression of PCAF induced cell apoptosis and repressed proliferation in Huh7 cells. (A) At the levels of both mRNA and protein, PCAF expression is increased by PCAF expressing plasmid in Huh7 cells; (B) Forced expression of PCAF induced a significant increase in apoptosis of Huh7 cells as assessed by staining with DAPI followed by fluorescence microscopy (P < 0.004); (C) The activity of the pro-apoptotic caspase 3 and 7 also showed up-regulated after ectopic expression of PCAF (P = 0.002); (D) Flow cytometry assay showed that the percents of early apoptosis cells and late apoptosis cells was increased 2–3 folds in Huh7 PCAF cells than in Huh7 Control cells(P = 0.002); (E) Cell proliferation as measured by BrdU incorporation was inhibited by forced expression of PCAF(P = 0.002); (F) As assessed by MTT assays, forced expression of PCAF was found to reduce viability of Huh7 cells at all four time points significantly.

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Molecular Cancer

ISSN: 1476-4598