Figure 6

Analysis of miRNA-regulated network of interacting partners involved various signaling pathways. A) Log₂ transformed FC values of 21 up regulated miRNAs were imported into the Core analysis tool of IPA software and a network for interacting miRNAs and their direct target proteins and proteins that target any of the up regulated miRNAs has been generated for 3wks CDX treated cells. Red symbols denote miRNAs or mature miRNAs. Proteins include growth factor/cytokines, enzymes, kinases, phosphatases, peptidases, translational regulator, transcriptional regulators, and transporters. B) The network in A is overlayed with disease relationship showing association of miRNA and protein interactions with various types of cancer. C and D) Regulatory network of the log₂ transformed FC values of 22 down regulated miRNAs and their target proteins in 3wks CDX treated cells (C) and overlay of disease association of the miRNA-protein regulatory network (D). Green symbols represent down regulated miRNAs and mature miRNAs. E and F) Regulatory network of log₂ transformed FC values of the up regulated miRNAs and their target proteins in cells treated with CSFBS for 3wks (E) and an overlay of disease relationship with the regulatory network in E. Red symbols represent miRNAs and mature miRNAs. G and H) Regulatory network of log₂ transformed FC values of the down regulated miRNAs and their target proteins in cells treated with CSFBS for 3wks (G) and an overlay of disease association with the regulatory network in G (H). Green symbols represent down regulated miRNAs and mature miRNAs.