Figure 9

Comparative analysis of target protein expression in LNCaP sublines in different treatment conditions. Western blot analysis of predicted target proteins regulated by down regulated miRNAs (shown in Table 3). Total proteins from all treated and untreated cells were used for immunoblot analysis using anti- FGD4 (A), ABHD3 (B), -DOK4 (C), VEGFA (D), and EGFR (E) antibodies. Upper panel shows representative images of the western blots. Bottom panel shows densitometric analysis of relation protein expression. Data shows mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, significance was calculated between LNCaP-104S untreated vs. specified treated cells.