Figure 1

AGK is upregulated in breast cancer. (A and B) Western blotting analysis of AGK expression in normal breast epithelial cells (NBECs) and 12 breast cancer cell lines (A) and in eight matched primary breast cancer tissues (T) and adjacent noncancerous tissues (N) (B). GAPDH was used as a loading control. (C) Immunohistochemical analysis of AGK protein expression in normal breast and primary tumor tissues. (D) Kaplan-Meier overall survival curves and univariate analyses (log-rank) comparing breast cancer patients with low (n = 103) and high (n = 100) AGK-expressing tumors (P < 0.001).