AGK promotes the tumorigenicity of breast cancer cells in vitro and in vivo . (A) Anchorage-independent growth assays of AGK-overexpressing cells and AGK-silenced cells. The number of colonies with a diameter larger than 0.1 mm was quantified after 10 days of culture. (B-D) Xenograft model in nude mice. MCF-7-AGK, MCF-7-AGK-RNAi and the respective control cells were inoculated into the fat-pad of nude mice (n = 5/group). (B): Representative images of tumor-bearing mice (left panel) and images of the tumors from all mice in each group (right panel). (C) Tumor volumes were measured on the indicated days. (D) Mean tumor weights. Each bar represents the mean ± SD of three independent experiments. *P < 0.05.