Figure 5

AGK alters expression in G1-S phase cell-cycle regulators. (A and B) Real-time PCR analysis of p21^Cip1, p27^Kip1 and cyclin D1 mRNA expression in AGK-infected cells (A) or AGK-shRNA infected cells (B). Gene expression levels were normalized to GAPDH. (C) Western blotting analysis of p21^Cip1, p27^Kip1, cyclin D1, p-Rb and total Rb protein expression in AGK-infected cells and AGK-shRNA infected cells; GAPDH was used as a loading control. Each bar represents the mean ± SD of three independent experiments. *P < 0.05.