Figure 3
From: The effects of diet induced obesity on breast cancer associated pathways in mice deficient in SFRP1
Genes involved in mammary gland development are aberrantly up-regulated in Sfrp1−/−mice in response to DIO. (A) Real-time PCR analysis of Wnt4 and Tnfs11 gene expression was carried out (n = 6/genotype). The results shown represent experiments performed in duplicate and are normalized to the amplification of β-Actin mRNA. Bars represent mean ± SEM of the difference in fold change compared with control ND fed mice. (B) Left panel, 3rd & 4th inguinal mammary gland sections were subjected to immunohistochemical analysis, stained for PR (brown chromogen), and representative images were captured at 400X are displayed for mice in each treatment group (scale bar 50 μm). Right panel, PR-stained cells were counted in each mammary gland (n = 6/genotype) and bars represent mean ± SEM % PR-positive cells. (*p < 0.05, significantly different from control mice fed a ND using Bonferroni’s t test after a two-way ANOVA).