Figure 1

DCQ reduces the viability and colony forming ability of breast cancer cell lines (MDA-MB-231 and MCF-7). (A) MTT viability assay was performed after 6 hours of exposure to DCQ under normoxia (21%) or hypoxia (1%). Results (Average ± SE) are from triplicate measurements from 3 independent experiments. One-way ANOVA was used to compare DCQ-treated versus control and statistical significance of p < 0.05 is indicated by *. Two sample t-test was used to compare between the effect of DCQ under normoxia versus hypoxia under the same concentration, p < 0.05 is indicated by †. (B,C) Clonogenic survival assay of cells exposed to different concentrations of DCQ under normoxia or hypoxia. Cells were treated with DCQ and incubated for 3 hours after which they were trypsinized and re-plated at a density of 300 cells/100 mm petri dish and surviving colonies (>50 cells) were counted 12 days after plating. *p < 0.05 significance with respect to control (one way ANOVA). †p < 0.05 significant difference between normoxia and hypoxia at the same DCQ concentration (two sample t-test). Results (Average ± SE) are from duplicate measurements of 3 independent experiments.