Figure 1

Regional interrogation of the DLEU2 gene including significantly differentially methylated probes identified by HM450 analysis. Bottom : Distribution of HM450 methylation probes across the DLEU2 region of 13q4. Genes located in this region include: DLEU1, DLEU2, miR15a/16-1 microRNA cluster, TRIM13, KCNRG and miR-3613. Top : HM450 probes identified as significantly differentially methylated between paediatric AML (FAB subtype M5) and non-leukaemic specimens have been plotted against genomic location. The leukaemic group (n = 16) refers to diagnostic bone marrow from paediatric patients. Non-leukaemic group (n = 11) consists of CD sorted cell populations (CD19+, CD33+, CD34+, CD45+) and patient remission specimens. Differential methylation is concentrated in the promoter region of the DLEU2/Alt1 long transcript, which also falls into the body region of DLEU1 within 3 CpG islands (Chr 13: 50, 690,000-50,708,000). Mean methylation β values and 95% CI are shown. Data for leukaemic samples are red and non-leukaemic samples in green. Methylation values range from 0 (0%, no detected methylation) to 1.0 (100% fully methylated). Significantly differentially methylated regions between leukaemic and non-leukaemic samples to p < 0.001 are highlighted with **.