Figure 2
From: MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress
OC125 weakly binds to cells that are generally considered negative for MUC16. SKOV-3 cells (identity confirmed by STR analysis) were stained with the primary (1°) antibodies OC125 or VK8 followed by incubation with allophycocyanine (APC)-labeled donkey anti-mouse (DAM) secondary antibody. Binding of the antibodies to SKOV-3 cells was determined on a LSR-II flow cytometer. RT-PCR using MUC16 primers previously reported [178], was used to determine expression of MUC16 in OVCAR-3, ECC-1 and SKOV-3 cells (data not shown).