Figure 1
From: A mechanically-induced colon cancer cell population shows increased metastatic potential
Multiple cancer cells lines show E-R transition on appropriate soft substrates. (a) HCT-116 cells cultured on 10 kPa PA gel substrates (coated with fibronectin) form cell colonies in 2–5 culture days. (b) HCT-116 cells begin to dissociate from colonies on the 10th day. On PS substrate or other stiffness PA gels under same culture condition, they do not show dissociation. (c) DU-145 cells cultured on 10 kPa PA gel substrates (coated with fibronectin) form cell colonies in 3–7 culture days. (d) DU-145 cells begin to dissociate from colonies on the 19th day. On PS substrate or other stiffness PA gels under same culture condition, they do not show dissociation. Scale bar: 100 μm.