IGF-I induced EMT and enhanced the migration ability in gastric cancer cells. MGC-803 and SGC-7901 cells were serum-starved overnight and then treated with or without 100 ng/mL of IGF-I for 48 h. (A) Photos were taken at × 20 magnification. (B) The cells were stained with antibodies to E-cadherin (green), Vimentin (red), and nuclei was stained with 4′,6′-diamidino-2-phenylindole (DAPI). Images were captured by fluorescence microscopy at × 40 magnification. (C) Cell lysates were collected for Western blot analysis. (D) The migration assays were performed using the Boyden chamber methods as described in Materials and methods. Data are means ± SD in three independent experiments. * IGF-I untreated vs. IGF-I treated, p < 0.05; E-cad, E-cadherin; Vim, Vimentin.